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BibTeX file of [Funke09] [show it without abstract]

@article{Funke09,
    author={Todd Funke and Yan Yang and Huijong Han and Martha Healy-Fried and Sanne Olesen and Andreas Becker and Ernst Sch\"onbrunn},
    title={Structural Basis of Glyphosate Resistance Resulting from the Double Mutation {Thr}$^{97}$ $\rightarrow$ {Ile} and {Pro}$^{101}$ $\rightarrow$ {Ser} in 5-Enolpyruvylshikimate-3-phosphate Synthase from \emph{Escherichia coli}},
    journal={J. Biol. Chem.},
    volume={284},
    pages={9854--9860},
    year={2009},
    keywords={KU; EPSPS;},
    url={https://doi.org/10.1074/jbc.M809771200},
    doi={10.1074/jbc.M809771200},
    abstract={The shikimate pathway enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) is the target of the broad spectrum herbicide glyphosate. The genetic engineering of EPSPS led to the introduction of glyphosate-resistant crops worldwide. The transgenic crops NK603 and GA21 carry two completely different versions of EPSPS. The CP4 EPSPS, the gene of which is expressed in NK603 crops, belongs to class II EPSPS, glyphosate-insensitive variants of this enzyme found in certain Gram-positive bacteria. GA21 corn, on the other hand, was created by point mutations of class I EPSPS, such as the enzymes from \emph{Zea mays} or \emph{Escherichia coli}, which are sensitive to low glyphosate concentrations. The structural basis of the glyphosate resistance resulting from these point mutations has remained obscure. We studied the kinetic and structural effects of the T97I/P101S double mutation, the molecular basis for GA21 corn, using EPSPS from \emph{E. coli}. The T97I/P101S enzyme is essentially insensitive to glyphosate ($K_i$=2.4 mM) but maintains high affinity for the substrate phosphoenolpyruvate (PEP) ($K_m$=0.1 mM). The crystal structure at 1.7-\AA resolution revealed that the dual mutation causes a shift of residue {Gly}$^{96}$ toward the glyphosate binding site, impairing efficient binding of glyphosate, while the side chain of {Ile}$^{97}$ points away from the substrate binding site, facilitating PEP utilization. The single site T97I mutation renders the enzyme sensitive to glyphosate and causes a substantial decrease in the affinity for PEP. Thus, only the concomitant mutations of {Thr}$^{97}$ and {Pro}$^{101}$ induce the conformational changes necessary to produce catalytically efficient, glyphosate-resistant class I EPSPS.
    
    } }



Todd Funke, Yan Yang, Huijong Han, Martha Healy-Fried, Sanne Olesen, Andreas Becker, and Ernst Schönbrunn, Structural Basis of Glyphosate Resistance Resulting from the Double Mutation Thr97 → Ile and Pro101 → Ser in 5-Enolpyruvylshikimate-3-phosphate Synthase from Escherichia coli, J. Biol. Chem. 284, 9854–9860 (2009) [pdf][pdf][abstract][abstract][link]doi:10.1074/jbc.M809771200


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