XFEL gets MAD
A combination between multi-wavelength anomalous diffraction (MAD) and x-ray
free-electron laser (XFEL) opens up a new route of structural determination.
Determination of protein structure is crucial to understand its biological
functions. X-ray crystallography has been widely used for this purpose, but it
suffers from two bottlenecks: the phase problem and growing high-quality
crystals. MAD is a well-established technique with synchrotron radiation to
address the phase problem, but standard synchrotron experiments need
large-scale crystals. XFEL is a promising tool for structural determination
without the need for large-scale crystals, but the phase problem remains
largely unsolved. Question is, can we do MAD with XFEL? Because a great deal of
ionization occurs during the extremely intense XFEL pulse, many have speculated
that the answer is no until now. Here, we demonstrate that the MAD equation
exists even with extensive electronic rearrangements at high x-ray intensity.
Our results shows that we can do MAD with XFEL. We then propose MAD phasing for
femtosecond x-ray nanocrystallography, which is one of the hottest topics in
Effective anomalous scattering factor spectra at high intensities.
The red curve is for the undamaged case, whereas the blue curve is for the highest intensity.
- Sang-Kil Son, Henry N. Chapman, and Robin Santra, Multiwavelength anomalous diffraction at high x-ray intensity, Phys. Rev. Lett. 107, 218102 (2011) [BibTeX][pdf][abstract]doi:10.1103/PhysRevLett.107.218102
- Sang-Kil Son, Henry N. Chapman, and Robin Santra, Determination of multiwavelength anomalous diffraction coefficients at high x-ray intensity, J. Phys. B: At. Mol. Opt. Phys. 46, 164015 (2013) [special issue on frontiers of free-electron laser science] [BibTeX][abstract]doi:10.1088/0953-4075/46/16/164015
- Lorenzo Galli, Sang-Kil Son, Thomas A. White, Robin Santra, Henry N. Chapman, and Max H. Nanao, Towards RIP using free electron laser SFX data, J. Synchrotron Rad. 22, 249255 (2015) [BibTeX][pdf][abstract]doi:10.1107/S1600577514027854
- Lorenzo Galli, Sang-Kil Son, M. Klinge, S. Bajt, A. Barty, R. Bean, C. Betzel, K. R. Beyerlein, C. Caleman, R. B. Doak, M. Duszenko, H. Fleckenstein, C. Gati, B. Hunt, R. A. Kirian, M. Liang, M. H. Nanao, K. Nass, D. Oberthür, L. Redecke, R. Shoeman, F. Stellato, C. H. Yoon, T. A. White, O. Yefanov, J. Spence, and H. N. Chapman, Electronic damage in S atoms in a native protein crystal induced by an intense x-ray free-electron laser pulse, Struct. Dyn. 2, 041703 (2015) [special issue on biology with x-ray lasers 2] [BibTeX][pdf][abstract]doi:10.1063/1.4919398
- Lorenzo Galli, Sang-Kil Son, Thomas R.M. Barends, Thomas A. White, Anton Barty, Sabine Botha, Sébastien Boutet, Carl Caleman, Bruce R. Doak, Max H. Nanao, Karol Nass, Robert L. Schoeman, Nicusor Timneanu, Robin Santra, Ilme Schlichting, and Henry N. Chapman, Towards phasing using high x-ray intensity, IUCrJ 2, 627634 (2015) [BibTeX][pdf][abstract]doi:10.1107/S2052252515014049
Multiwavelength anomalous diffraction at high x-ray intensity by Sang-Kil Son
- New Science Opportunities at FLASH (DESY, Hamburg, Germany, October 12-14, 2011) [oral presentation] [BibTeX][pdf][slide: 8Mb][abstract][link]
- DPG Spring Meeting of the Section AMOP (German Physical Society, Stuttgart, Germany, March 12-16, 2012) [oral presentation] [BibTeX][slide: 4Mb][abstract][link]
- Ultrafast Dynamic Imaging of Matter (Banff, Canada, July 1-3, 2012) [oral presentation] [BibTeX][pdf][slide: 6Mb][abstract][link]
- The 24th PLS Synchrotron Radiation Users' Workshop (POSTECH, Pohang, Korea, November 22, 2012) [invited talk] [BibTeX][slide: 7Mb][abstract][link]
- Workshop on Resonant Elastic X-ray Scattering in Condensed Matter (Oxford, UK, July 15-19, 2013) [invited talk] [BibTeX][pdf][slide: 10Mb][abstract][link]
- Photoionization and Photodetachment (Gordon Research Conference, Galveston, Texas, February 23-28, 2014) [invited talk] [BibTeX][link]
Multiwavelength anomalous diffraction at high x-ray intensity by Sang-Kil Son, Henry N. Chapman, and Robin Santra
- European XFEL and HASYLAB Users' Meeting (DESY, Hamburg, Germany, January 25-27, 2012) [poster] [BibTeX][poster: 4Mb][abstract][link]
- Science at FELs (DESY, Hamburg, Germany, July 15-18, 2012) [poster] [BibTeX][pdf][abstract][link]