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Abstract of [Vakili22a]

Mix-and-extrude using 3D printed nozzles for time-resolved membrane protein crystallography

Mohammad Vakili, Huijong Han, Christina Schmidt, Agnieszka Wrona, Marco Kloos, Iñaki de Diego, Katerina Dörner, Joana Valerio, Ekaterina Round, Kristina Lorenzen, and Joachim Schulz

(preprint, 2022)

[bib][BibTeX][link]doi:10.1101/2022.11.23.517685

Time-resolved crystallography enabled the visualization of protein molecular motion during reaction. Currently, light is the most prevalent reaction initiator, but the number of light-activated proteins is extremely limited. Instead, more biological reactions are triggered by interaction with ligands. To examine the molecular action of these mixing-initiated reactions by X-ray diffraction, a sample delivery method that allows for diffusive mixing of crystals with ligands in a short amount of time corresponding to the reaction time to be studied is needed. This paper describes the mix-and-extrude nozzle for delivering membrane proteins crystallized in lipid cubic phase and ligands, along with its preliminary characterization and outlook for future developments.

Tags: HVE, LCP, European XFEL


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