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BibTeX file of [Doppler26] [show it without abstract]

@article{Doppler26,
    author={Diandra Doppler and Alice Grieco and Domin Koh and Abhik Manna and Adil Ansari and Roberto Alvarez and Konstantinos Karpos and Hung Le and Mukul Sonker and Gihan K. Ketawala and Samira Mahmud and Isabel Quereda-Moraleda and Sayantee Sen and Angel L. Pey and Romain Letrun and Katerina D{\"o}rner and Jayanath C. P. Koliyadu and Raphael de Wijn and Johan Bielecki and Huijong Han and Chan Kim and Faisal H. M. Koua and Adam Round and Abhisakh Sarma and Tokushi Sato and Christina Schmidt and Mohammad Vakili and Dmitrii Zabelskii and Richard Bean and Adrian P. Mancuso and Joachim Schulz and Raimund Fromme and Milagros Medina and Thomas D. Grant and Petra Fromme and Richard A. Kirian and Sabine Botha and Jose Manuel Martin-Garcia and Alexandra Ros},
    title={Minimized sample consumption for time-resolved serial crystallography applied to the redox cycle of human {NQO1}},
    journal={Commun. Chem.},
    volume={9},
    pages={107},
    year={2026},
    keywords={SFX; serial crystallography; TR-SFX; mix-and-inject; MISC; European XFEL;},
    url={https://doi.org/10.1038/s42004-026-01908-9},
    doi={10.1038/s42004-026-01908-9},
    abstract={Sample consumption for serial femtosecond crystallography with X-ray free electron lasers remains a major limitation preventing broader use in macromolecular crystallography. This drawback is exacerbated in time-resolved (TR) experiments, where the amount of sample required per reaction time point is multiplied by the number of time points investigated. To reduce this limitation, we demonstrate a segmented droplet generation strategy coupled to a mix-and-inject approach for TR studies at the European XFEL. The injector produces synchronized droplet trains that enable stable and reproducible injection of protein crystal slurries at significantly reduced flow rates. Using the human flavoenzyme NAD(P)H:quinone oxidoreductase 1 (NQO1) as a test system, we collected diffraction data after mixing with NADH at 0.3~s and 1.2~s delays. The segmented injection approach achieved up to 97% reduction in sample consumption compared with continuous-flow injection while maintaining data quality suitable for TR crystallography. Reproducible electron density features consistent with low-occupancy NADH binding illustrate both the feasibility and the current limits of studying dynamic redox enzymes using this approach. This work establishes segmented droplet generation as a sample-efficient and XFEL-compatible method for future time-resolved serial crystallography experiments.} }

Diandra Doppler, Alice Grieco, Domin Koh, Abhik Manna, Adil Ansari, Roberto Alvarez, Konstantinos Karpos, Hung Le, Mukul Sonker, Gihan K. Ketawala, Samira Mahmud, Isabel Quereda-Moraleda, Sayantee Sen, Angel L. Pey, Romain Letrun, Katerina Dörner, Jayanath C.P. Koliyadu, Raphael de Wijn, Johan Bielecki, Huijong Han, Chan Kim, Faisal H.M. Koua, Adam Round, Abhisakh Sarma, Tokushi Sato, Christina Schmidt, Mohammad Vakili, Dmitrii Zabelskii, Richard Bean, Adrian P. Mancuso, Joachim Schulz, Raimund Fromme, Milagros Medina, Thomas D. Grant, Petra Fromme, Richard A. Kirian, Sabine Botha, Jose Manuel Martin-Garcia, and Alexandra Ros, Minimized sample consumption for time-resolved serial crystallography applied to the redox cycle of human NQO1, Commun. Chem. 9, 107 (2026) [pdf][pdf][abstract][abstract][link]doi:10.1038/s42004-026-01908-9


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